Purification and Characteristics of Sorbitol-6-phosphate Dehydrogenase from Loquat Leaves

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Purification and characterization of sorbitol-6-phosphate phosphatase from apple leaves

Sorbitol-6-phosphate phosphatase (SorPP; EC 3.1.3.50) catalyzes the final step in sorbitol biosynthesis in sorbitol-synthesizing plant species, but its kinetic and regulatory properties have not been characterized. In this study, the enzyme was purified 1727-fold to apparent homogeneity from apple leaves with a maximal specific activity of 89.8 mmol min 1 mg 1 protein measured at 2 mM sorbitol-...

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D-sorbitol-6-phosphate dehydrogenase from Lactobacillus casei.

Several different systems for the conversion of sorbitol to hexose have been described in microorganisms. In the pseudomonads, sorbitol may be oxidized to fructose (Shaw, 1956) or to sorbose (Sebek and Randles, 1952). The latter investigators concluded that the conversion of sorbitol to fructose or sorbose did not involve phosphorylation since carbon dioxide was not liberated from bicarbonate b...

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D-Mannitol 1-phosphate dehydrogenase and D-sorbitol 6-phosphate dehydrogenase in Aerobacter aerogenes.

n-Mannitol has been used as a carbon source for the growth of many microorganisms. Some bacteria contain diphosphopyridine nucleotide-dependent dehydrogenases that will oxidize n-mannitol to n-fructose at the nonphosphorylated level (2-5). On the other hand, Wolff and Kaplan (6, 7) have reported the presence of a diphosphopyridine nucleotide-dependent n-mannitol l-phosphate dehydrogenase that o...

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D-Mannitol l-Phosphate Dehydrogenase and D-Sorbitol 6-Phosphate Dehydrogenase in Aerobacter aerogenes”

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Relationship between sorbitol and loquat

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1981

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.67.2.221